› 5yy2

X-ray diffraction
2.91Å resolution

Crystal structure of AsqI with Zn

Released:
DOI: 10.2210/pdb5yy2/pdb
PDB entry 5yy2 coloured by chain, front view.
PDB entry 5yy2 coloured by chain, side view.
PDB entry 5yy2 coloured by chain, top view.
Source organism: Aspergillus nidulans FGSC A4
Primary publication:
Enzymatic one-step ring contraction for quinolone biosynthesis.
Kishimoto S, Hara K, Hashimoto H, Hirayama Y, Champagne PA, Houk KN, Tang Y, Watanabe K
OpenAccess logo Nat Commun 9 2826 (2018)
PMID: 30026518

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-504531 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclopenase asqI Chain: A
Molecule details ›
Chain: A
Length: 749 amino acids
Theoretical weight: 85.64 KDa
Source organism: Aspergillus nidulans FGSC A4
Expression system: Escherichia coli
UniProt:
  • Canonical: C8VJQ3 (Residues: 1-14, 15-731; Coverage: 100%)
Gene names: AN9228, ANIA_11193, asqI
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: I222
Unit cell:
a: 84.582Å b: 114.858Å c: 157.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.215
Expression system: Escherichia coli

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Citations

2 review citations

Alkaloids from Marine Fungi: Promising Antimicrobials.
Willems et al. (2020)
1 more

1 mention without citation

Enzymatic one-step ring contraction for quinolone biosynthesis.
Kishimoto et al. (2018)