› 5z08

X-ray diffraction
2.2Å resolution

The crystal structure of kinetochore subunits Cenp-H/I/K triple complex

Released:
DOI: 10.2210/pdb5z08/pdb
PDB entry 5z08 coloured by chain, front view.
PDB entry 5z08 coloured by chain, side view.
PDB entry 5z08 coloured by chain, top view.
Source organisms:
Primary publication:
Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment.
Hu L, Huang H, Hei M, Yang Y, Li S, Liu Y, Dou Z, Wu M, Li J, Wang GZ, Yao X, Liu H, He X, Tian W
OpenAccess logo Nucleic Acids Res 47 468-479 (2019)
PMID: 30407575

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124105 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cenp-I Chains: A, B
Molecule details ›
Chains: A, B
Length: 229 amino acids
Theoretical weight: 25.79 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Escherichia coli
UniProt:
  • Canonical: G0SFF7 (Residues: 1-229; Coverage: 34%)
Gene name: CTHT_0061880
Sequence domains: Mis6
Centromere protein H C-terminal domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 168 amino acids
Theoretical weight: 18.87 KDa
Source organism: Thermothielavioides terrestris NRRL 8126
Expression system: Escherichia coli
UniProt:
  • Canonical: G2R3T1 (Residues: 161-328; Coverage: 51%)
Gene name: THITE_2065850
Sequence domains: Centromere-associated protein K
Centromere protein H C-terminal domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 45 amino acids
Theoretical weight: 5.2 KDa
Source organism: Thermothielavioides terrestris NRRL 8126
Expression system: Escherichia coli
UniProt:
  • Canonical: G2R207 (Residues: 184-228; Coverage: 20%)
Gene name: THITE_2113209

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P41
Unit cell:
a: 67.847Å b: 67.847Å c: 186.269Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.23
Expression system: Escherichia coli

Quick links

Citations

2 review citations

E3 Ubiquitin Ligase TRIM Proteins, Cell Cycle and Mitosis.
Venuto et al. (2019)
1 more

4 mentions without citation

Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome.
Yan et al. (2019)
3 more