› 5z12

X-ray diffraction
2.75Å resolution

A structure of FXR/RXR

Released:
DOI: 10.2210/pdb5z12/pdb
PDB entry 5z12 coloured by chain, front view.
PDB entry 5z12 coloured by chain, side view.
PDB entry 5z12 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the heterodimeric complex of the nuclear receptors FXR and RXR.
Zheng W, Lu Y, Tian S, Ma F, Wei Y, Xu S, Li Y
J Biol Chem 293 12535-12541 (2018)
PMID: 29934308

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-539263 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Bile acid receptor Chains: A, D
Molecule details ›
Chains: A, D
Length: 228 amino acids
Theoretical weight: 26.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96RI1 (Residues: 259-486; Coverage: 47%)
Gene names: BAR, FXR, HRR1, NR1H4, RIP14
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Retinoic acid receptor RXR-alpha Chains: B, C
Molecule details ›
Chains: B, C
Length: 231 amino acids
Theoretical weight: 25.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19793 (Residues: 228-458; Coverage: 50%)
Gene names: NR2B1, RXRA
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Nuclear receptor coactivator 2 Chains: F, I
Molecule details ›
Chains: F, I
Length: 9 amino acids
Theoretical weight: 1.16 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 687-695; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2
Nuclear receptor coactivator 2 Chains: H, J
Molecule details ›
Chains: H, J
Length: 6 amino acids
Theoretical weight: 807 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 687-692; Coverage: 0%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

2 bound ligands:
Ligand 33Y 2 x 33Y
Ligand 9CR 2 x 9CR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 89.873Å b: 95.484Å c: 116.721Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.23 0.29
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

Nuclear receptor crosstalk - defining the mechanisms for therapeutic innovation.
De Bosscher et al. (2020)
11 more

4 mentions without citation

FXR: structures, biology, and drug development for NASH and fibrosis diseases.
Tian et al. (2022)
3 more