› 5z24

X-ray diffraction
2.4Å resolution

Crystal structure of shaft pilin spaD from Lactobacillus rhamnosus GG - K365A mutant

Released:
DOI: 10.2210/pdb5z24/pdb
PDB entry 5z24 coloured by chain, front view.
PDB entry 5z24 coloured by chain, side view.
PDB entry 5z24 coloured by chain, top view.
Source organism: Lacticaseibacillus rhamnosus GG
Primary publication:
Bent conformation of a backbone pilin N-terminal domain supports a three-stage pilus assembly mechanism.
Chaurasia P, Pratap S, Palva A, von Ossowski I, Krishnan V
OpenAccess logo Commun Biol 1 94 (2018)
PMID: 30271975

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166353 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pilus assembly protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 465 amino acids
Theoretical weight: 50.98 KDa
Source organism: Lacticaseibacillus rhamnosus GG
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 50.821Å b: 74.886Å c: 403.652Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.241 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

SpaB, an atypically adhesive basal pilin from the lactobacillar SpaCBA pilus: crystallization and X-ray diffraction analysis.
Kumar Megta et al. (2019)
4 more

1 mention without citation

Bent conformation of a backbone pilin N-terminal domain supports a three-stage pilus assembly mechanism.
Chaurasia et al. (2018)