› 5zb8

X-ray diffraction
2.5Å resolution

Crystal structure of the novel lesion-specific endonuclease PfuEndoQ from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb5zb8/pdb
PDB entry 5zb8 coloured by chain, front view.
PDB entry 5zb8 coloured by chain, side view.
PDB entry 5zb8 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Crystal structure of the novel lesion-specific endonuclease PfuEndoQ from Pyrococcus furiosus.
Miyazono KI, Ishino S, Makita N, Ito T, Ishino Y, Tanokura M
OpenAccess logo Nucleic Acids Res 46 4807-4818 (2018)
PMID: 29660024

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-570347 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TIGR00375 family protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 400 amino acids
Theoretical weight: 44.97 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U0N5 (Residues: 3-400; Coverage: 94%)
Gene name: PF1551

Ligands and Environments

2 bound ligands:
Ligand ZN 15 x ZN
Ligand SM 7 x SM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 257.354Å b: 82.245Å c: 116.581Å
α: 90° β: 109.13° γ: 90°
R-values:
R R work R free
0.178 0.176 0.209
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Archaeal DNA Repair Mechanisms.
Marshall et al. (2020)
2 more

3 mentions without citation

Crystal structure of the novel lesion-specific endonuclease PfuEndoQ from Pyrococcus furiosus.
Miyazono et al. (2018)
2 more