› 5zef

X-ray diffraction
2.01Å resolution

Crystal structure of Entamoeba histolytica Arginase in complex with L- Norvaline at 2.01 A

Released:
DOI: 10.2210/pdb5zef/pdb
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PDB entry 5zef coloured by chain, front view.
PDB entry 5zef coloured by chain, side view.
PDB entry 5zef coloured by chain, top view.
Source organism: Entamoeba histolytica
Primary publication:
Structural insights into Entamoeba histolytica arginase and structure-based identification of novel non-amino acid based inhibitors as potential antiamoebic molecules.
Malik A, Dalal V, Ankri S, Tomar S
FEBS J 286 4135-4155 (2019)
PMID: 31199070

Function and Biology Details

Reaction catalysed: 
L-arginine + H(2)O = L-ornithine + urea
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arginase Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 34.83 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4LSS0 (Residues: 1-296; Coverage: 100%)
Gene names: ARG, EHI_152330
Sequence domains: Arginase family

Ligands and Environments

4 bound ligands:
Ligand NVA 2 x NVA
Ligand MN 4 x MN
Ligand GOL 2 x GOL
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I212121
Unit cell:
a: 87.947Å b: 98.358Å c: 135.329Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.21
Expression system: Escherichia coli

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Citations

6 citation in other articles

Computational guided identification of novel potent inhibitors of N-terminal domain of nucleocapsid protein of severe acute respiratory syndrome coronavirus 2.
Dhankhar et al. (2022)
5 more

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