› 5zfr

X-ray diffraction
3.1Å resolution

Crystal structure of PilB, an extension ATPase motor of Type IV pilus, from Geobacter sulfurreducens

Released:
DOI: 10.2210/pdb5zfr/pdb
PDB entry 5zfr coloured by chain, front view.
PDB entry 5zfr coloured by chain, side view.
PDB entry 5zfr coloured by chain, top view.
Source organism: Geobacter sulfurreducens PCA
Primary publication:
Structural insights into the mechanism of Type IVa pilus extension and retraction ATPase motors.
Solanki V, Kapoor S, Thakur KG
FEBS J 285 3402-3421 (2018)
PMID: 30066435

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-565215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacterial type II secretion system protein E domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 582 amino acids
Theoretical weight: 64.35 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli
UniProt:
  • Canonical: Q74D28 (Residues: 1-568; Coverage: 100%)
Gene names: GSU1491, pilB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 176.301Å b: 176.301Å c: 138.561Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.218 0.247
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Type IV pili: dynamics, biophysics and functional consequences.
Craig et al. (2019)
3 more

2 mentions without citation

Structural insights into the mechanism of Type IVa pilus extension and retraction ATPase motors.
Solanki et al. (2018)
1 more