› 5zih

X-ray diffraction
2.6Å resolution

Crystal structure of the red light-activated channelrhodopsin Chrimson.

Released:
DOI: 10.2210/pdb5zih/pdb
PDB entry 5zih coloured by chain, front view.
PDB entry 5zih coloured by chain, side view.
PDB entry 5zih coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Crystal structure of the red light-activated channelrhodopsin Chrimson.
Oda K, Vierock J, Oishi S, Rodriguez-Rozada S, Taniguchi R, Yamashita K, Wiegert JS, Nishizawa T, Hegemann P, Nureki O
OpenAccess logo Nat Commun 9 3949 (2018)
PMID: 30258177

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185252 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensory opsin A Chains: A, B
Molecule details ›
Chains: A, B
Length: 347 amino acids
Theoretical weight: 38.96 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8L435 (Residues: 1-346; Coverage: 47%)
Gene name: CSOA
Sequence domains: Bacteriorhodopsin-like protein

Ligands and Environments

1 bound ligand:
Ligand OLC 19 x OLC
1 modified residue:
Ligand LYR 2 x LYR

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P212121
Unit cell:
a: 60.99Å b: 81.44Å c: 170.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.228 0.279
Expression system: Spodoptera frugiperda

Quick links

Citations

13 review citations

Principles of Optogenetic Methods and Their Application to Cardiac Experimental Systems.
Ferenczi et al. (2019)
12 more

7 mentions without citation

Machine learning-guided channelrhodopsin engineering enables minimally invasive optogenetics.
Bedbrook et al. (2019)
6 more