› 5zjb

X-ray diffraction
1.7Å resolution

Structure of N-acetylmannosamine-6-phosphate-2-epimerase from Vibrio cholerae

Released:
DOI: 10.2210/pdb5zjb/pdb
PDB entry 5zjb coloured by chain, front view.
PDB entry 5zjb coloured by chain, side view.
PDB entry 5zjb coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Crystal structures and kinetic analyses of N-acetylmannosamine-6-phosphate 2-epimerases from Fusobacterium nucleatum and Vibrio cholerae.
Manjunath L, Guntupalli SR, Currie MJ, North RA, Dobson RCJ, Nayak V, Subramanian R
Acta Crystallogr F Struct Biol Commun 74 431-440 (2018)
PMID: 29969107

Function and Biology Details

Reaction catalysed: 
N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative N-acetylmannosamine-6-phosphate 2-epimerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 27.1 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KR62 (Residues: 6-240; Coverage: 98%)
Gene names: VC_1781, nanE
Sequence domains: Putative N-acetylmannosamine-6-phosphate epimerase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand MLI 3 x MLI
Ligand PEG 5 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 49.325Å b: 70.417Å c: 150.399Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.179 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Glycan cross-feeding supports mutualism between Fusobacterium and the vaginal microbiota.
Agarwal et al. (2020)

2 mentions without citation

Crystal structures and kinetic analyses of N-acetylmannosamine-6-phosphate 2-epimerases from Fusobacterium nucleatum and Vibrio cholerae.
Manjunath et al. (2018)
1 more