› 5zq7

X-ray diffraction
2.85Å resolution

SidE-Ubi-NAD

Released:
DOI: 10.2210/pdb5zq7/pdb
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PDB entry 5zq7 coloured by chain, front view.
PDB entry 5zq7 coloured by chain, side view.
PDB entry 5zq7 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Insights into Non-canonical Ubiquitination Catalyzed by SidE.
Wang Y, Shi M, Feng H, Zhu Y, Liu S, Gao A, Gao P
Cell 173 1231-1243.e16 (2018)
PMID: 29731171

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143435 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SidE Chains: A, C
Molecule details ›
Chains: A, C
Length: 845 amino acids
Theoretical weight: 96.81 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6BBR6 (Residues: 240-1076; Coverage: 55%)
Gene name: laiD
Sequence domains:
Ubiquitin Chains: B, D
Molecule details ›
Chains: B, D
Length: 79 amino acids
Theoretical weight: 8.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family

Ligands and Environments

2 bound ligands:
Ligand NAD 2 x NAD
Ligand AMP 2 x AMP
1 modified residue:
Ligand MSE 34 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 93.409Å b: 129.381Å c: 192.381Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.239
Expression system: Escherichia coli

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Citations

18 review citations

The role of ubiquitination in tumorigenesis and targeted drug discovery.
Deng et al. (2020)
17 more

1 mention without citation

Uncovering the Structural Basis of a New Twist in Protein Ubiquitination.
Puvar et al. (2019)

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