› 5zs6

X-ray diffraction
2.81Å resolution

Dodecameric structure of a small Heat Shock Protein from Mycobacterium marinum M: Form-2

Released:
DOI: 10.2210/pdb5zs6/pdb
PDB entry 5zs6 coloured by chain, front view.
PDB entry 5zs6 coloured by chain, side view.
PDB entry 5zs6 coloured by chain, top view.
Source organism: Mycobacterium marinum M
Primary publication:
Dodecameric structure of a small heat shock protein from Mycobacterium marinum M.
Bhandari S, Biswas S, Chaudhary A, Dutta S, Suguna K
Proteins 87 365-379 (2019)
PMID: 30632633

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109557 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SHSP domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.54 KDa
Source organism: Mycobacterium marinum M
Expression system: Escherichia coli
UniProt:
  • Canonical: B2HF11 (Residues: 1-149; Coverage: 100%)
Gene name: MMAR_2991
Sequence domains: Hsp20/alpha crystallin family
GLY-ARG-LEU-LEU Chain: U
Molecule details ›
Chain: U
Length: 4 amino acids
Theoretical weight: 459 Da
Source organism: Mycobacterium marinum M
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand PEG 3 x PEG
Ligand PRO 1 x PRO
Ligand CL 2 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: I23
Unit cell:
a: 91.29Å b: 91.29Å c: 91.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.199 0.25
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Is the lipochaperone activity of sHSP a key to the stress response encoded in its primary sequence?
Bellanger et al. (2023)
3 other articles