› 5ztl

X-ray diffraction
1.85Å resolution

Non-cryogenic structure of light-driven chloride pump having an NTQ motif

Released:
DOI: 10.2210/pdb5ztl/pdb
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PDB entry 5ztl coloured by chain, front view.
PDB entry 5ztl coloured by chain, side view.
PDB entry 5ztl coloured by chain, top view.
Source organism: Nonlabens marinus
Primary publication:
Non-cryogenic structure of a chloride pump provides crucial clues to temperature-dependent channel transport efficiency.
Yun JH, Li X, Park JH, Wang Y, Ohki M, Jin Z, Lee W, Park SY, Hu H, Li C, Zatsepin N, Hunter MS, Sierra RG, Koralek J, Yoon CH, Cho HS, Weierstall U, Tang L, Liu H, Lee W
J Biol Chem 294 794-804 (2019)
PMID: 30455349

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chloride pumping rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 30.49 KDa
Source organism: Nonlabens marinus
Expression system: Escherichia coli
UniProt:
  • Canonical: W8VZW3 (Residues: 1-272; Coverage: 100%)
Gene names: ClR, NMS_1267
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand RET 1 x RET
Ligand OLA 7 x OLA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: C2
Unit cell:
a: 103.36Å b: 50.09Å c: 69.4Å
α: 90° β: 109.65° γ: 90°
R-values:
R R work R free
0.242 0.24 0.278
Expression system: Escherichia coli

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Citations

4 review citations

Diversity, Mechanism, and Optogenetic Application of Light-Driven Ion Pump Rhodopsins.
Inoue K. (2021)
3 more

2 mentions without citation

Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers.
Hosaka et al. (2022)
1 more

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