5zx2

X-ray diffraction
2.8Å resolution

Mycobacterium tuberculosis RNA polymerase transcription initiation complex with ECF sigma factor sigma H and 7nt RNA

Released:
Model geometry
Fit model/data

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-119536 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 40.28 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WGZ1 (Residues: 1-347; Coverage: 100%)
Gene names: MTCY13E12.10c, Rv3457c, rpoA
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1174 amino acids
Theoretical weight: 129.6 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WGY9 (Residues: 7-1178; Coverage: 100%)
Gene names: MTCI376.08c, Rv0667, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
DNA-directed RNA polymerase subunit omega Chain: E
ECF RNA polymerase sigma factor SigH Chain: F
DNA (48-MER) Chain: G
DNA (47-MER) Chain: H
RNA (5'-R(*CP*CP*CP*UP*CP*GP*A)-3') Chain: I

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 129.824Å b: 164.027Å c: 214.792Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.22 0.255
Expression system: Escherichia coli BL21(DE3)