› 5zyq

X-ray diffraction
2.53Å resolution

The Structure of Human PAF1/CTR9 complex

Released:
DOI: 10.2210/pdb5zyq/pdb
PDB entry 5zyq coloured by chain, front view.
PDB entry 5zyq coloured by chain, side view.
PDB entry 5zyq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Paf1 and Ctr9 subcomplex formation is essential for Paf1 complex assembly and functional regulation.
Xie Y, Zheng M, Chu X, Chen Y, Xu H, Wang J, Zhou H, Long J
OpenAccess logo Nat Commun 9 3795 (2018)
PMID: 30228257

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180142 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase II-associated factor 1 homolog; RNA polymerase-associated protein CTR9 homolog Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 36.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6PD62 (Residues: 1-249; Coverage: 21%)
  • Canonical: Q8N7H5 (Residues: 57-116; Coverage: 11%)
Gene names: CTR9, KIAA0155, PAF1, PD2, SH2BP1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P3112
Unit cell:
a: 82.415Å b: 82.415Å c: 145.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.202 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Paf1 Complex: A Keystone of Nuclear Regulation Operating at the Interface of Transcription and Chromatin.
Francette et al. (2021)
1 more

1 mention without citation

Enhancing HIV-1 latency reversal through regulating the elongating RNA Pol II pause-release by a small-molecule disruptor of PAF1C.
Soliman et al. (2023)