› 6a65

X-ray diffraction
1.77Å resolution

Placental protein 13/galectin-13 variant R53HR55N with Tris

Released:
DOI: 10.2210/pdb6a65/pdb
PDB entry 6a65 coloured by chain, front view.
PDB entry 6a65 coloured by chain, side view.
PDB entry 6a65 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Resetting the ligand binding site of placental protein 13/galectin-13 recovers its ability to bind lactose.
Su J, Cui L, Si Y, Song C, Li Y, Yang T, Wang H, Mayo KH, Tai G, Zhou Y
OpenAccess logo Biosci Rep 38 (2018)
PMID: 30413611

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-577445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactoside-binding soluble lectin 13 Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 15.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHV8 (Residues: 2-139; Coverage: 99%)
Gene names: LGALS13, PLAC8
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C222
Unit cell:
a: 57.316Å b: 91.699Å c: 50.339Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.201 0.254
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Structural Biology of Galectin-Ligand Recognition: Current Advances in Modeling Tools, Protein Engineering, and Inhibitor Design.
Modenutti et al. (2019)
2 more

1 mention without citation

Resetting the ligand binding site of placental protein 13/galectin-13 recovers its ability to bind lactose.
Su et al. (2018)