› 6ajw

X-ray diffraction
1.4Å resolution

Crystal structure of BRD4 in complex with DMSO (Cocktail No. 4)

Released:
DOI: 10.2210/pdb6ajw/pdb
PDB entry 6ajw coloured by chain, front view.
PDB entry 6ajw coloured by chain, side view.
PDB entry 6ajw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and thermodynamic characterization of the binding of isoliquiritigenin to the first bromodomain of BRD4.
Yokoyama T, Matsumoto K, Ostermann A, Schrader TE, Nabeshima Y, Mizuguchi M
FEBS J 286 1656-1667 (2019)
PMID: 30565859

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bromodomain-containing protein 4 Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 16.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60885 (Residues: 42-168; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain

Ligands and Environments

2 bound ligands:
Ligand NA 3 x NA
Ligand DMS 1 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 33.407Å b: 47.152Å c: 78.316Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.228
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Flavonoids against the SARS-CoV-2 induced inflammatory storm.
Liskova et al. (2021)
1 more