6c23

Electron Microscopy
3.9Å resolution

Cryo-EM structure of PRC2 bound to cofactors AEBP2 and JARID2 in the Compact Active State

Released:
Source organism: Homo sapiens
Related structures: EMD-7334

Function and Biology Details

Reaction catalysed:
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-131024 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Polycomb protein SUZ12 Chains: A, M, Q
Molecule details ›
Chains: A, M, Q
Length: 739 amino acids
Theoretical weight: 83.18 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q15022 (Residues: 1-739; Coverage: 100%)
Gene names: CHET9, JJAZ1, KIAA0160, SUZ12
Sequence domains: VEFS-Box of polycomb protein
Protein Jumonji Chain: E
Molecule details ›
Chain: E
Length: 348 amino acids
Theoretical weight: 37.94 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q92833 (Residues: 106-450; Coverage: 28%)
Gene names: JARID2, JMJ
Histone-lysine N-methyltransferase EZH2 Chains: C, K
Polycomb protein EED Chain: L
Histone-binding protein RBBP4 Chain: N
JARID2-substrate Chain: O
Zinc finger protein AEBP2 Chain: P
SUZ12 Chain: Z
Protein Jumonji Chain: B

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.9Å
Relevant EMDB volumes: EMD-7334
Expression system: Trichoplusia ni