6ea7

X-ray diffraction
4.25Å resolution

Structure of EBOV GPcl in complex with the pan-ebolavirus mAb ADI-15878

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-232129 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (7 distinct):
GP1 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 163 amino acids
Theoretical weight: 17.78 KDa
Source organism: Ebola virus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q05320 (Residues: 32-194; Coverage: 25%)
Gene name: GP
Sequence domains: Filovirus glycoprotein
GP2 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 111 amino acids
Theoretical weight: 12.35 KDa
Source organism: Ebola virus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q05320 (Residues: 502-612; Coverage: 17%)
Gene name: GP
Sequence domains: Envelope glycoprotein GP2-like, HR1-HR2
ADI-15878 Fab Heavy Chain Chains: H, M, Q
Molecule details ›
Chains: H, M, Q
Length: 233 amino acids
Theoretical weight: 24.74 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
ADI-15878 Fab Light Chain Chains: L, N, R
Molecule details ›
Chains: L, N, R
Length: 213 amino acids
Theoretical weight: 23.09 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P3121
Unit cell:
a: 152.781Å b: 152.781Å c: 247.685Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.283 0.28 0.324
Expression system: Drosophila melanogaster