› 6eo5

X-ray diffraction
2.6Å resolution

Physcomitrella patens BBE-like 1 variant D396N

Released:
DOI: 10.2210/pdb6eo5/pdb
PDB entry 6eo5 coloured by chain, front view.
PDB entry 6eo5 coloured by chain, side view.
PDB entry 6eo5 coloured by chain, top view.
Source organism: Physcomitrium patens
Primary publication:
The single berberine bridge enzyme homolog of Physcomitrella patens is a cellobiose oxidase.
Toplak M, Wiedemann G, Ulićević J, Daniel B, Hoernstein SNW, Kothe J, Niederhauser J, Reski R, Winkler A, Macheroux P
OpenAccess logo FEBS J 285 1923-1943 (2018)
PMID: 29633551

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-496707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding PCMH-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 479 amino acids
Theoretical weight: 52.62 KDa
Source organism: Physcomitrium patens
Expression system: Komagataella phaffii
UniProt:
  • Canonical: A0A2K1JP57 (Residues: 31-501; Coverage: 100%)
Gene name: PHYPA_015719
Sequence domains:
Structure domains: Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase; domain 3

Ligands and Environments

2 bound ligands:
Ligand FAD 2 x FAD
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P41212
Unit cell:
a: 148.67Å b: 148.67Å c: 204.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.21
Expression system: Komagataella phaffii

Quick links

Citations

1 review citation

Dampening the DAMPs: How Plants Maintain the Homeostasis of Cell Wall Molecular Patterns and Avoid Hyper-Immunity.
Pontiggia et al. (2020)
4 other articles

1 mention without citation

The single berberine bridge enzyme homolog of Physcomitrella patens is a cellobiose oxidase.
Toplak et al. (2018)