› 6fua

X-ray diffraction
2.8Å resolution

ATP phosphoribosyltransferase (HisZG ATPPRT) from Psychrobacter arcticus in complex with PRPP and ADP

Released:
DOI: 10.2210/pdb6fua/pdb
PDB entry 6fua coloured by chain, front view.
PDB entry 6fua coloured by chain, side view.
PDB entry 6fua coloured by chain, top view.
Source organism: Psychrobacter arcticus 273-4
Primary publication:
Catalytic and Anticatalytic Snapshots of a Short-Form ATP Phosphoribosyltransferase
Alphey MS, Fisher G, Hirschi JS, Stroek R, Ge Y, Gould ER, Czekster CM, Liu H, Florence GJ, Vetticatt MJ, Naismith JH, da Silva RG
ACS Catalysis (2018)

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-559823 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP phosphoribosyltransferase regulatory subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.13 KDa
Source organism: Psychrobacter arcticus 273-4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4FTX3 (Residues: 1-387; Coverage: 100%)
Gene names: Psyc_0676, hisZ
Sequence domains: Histidyl-tRNA synthetase
ATP phosphoribosyltransferase Chains: C, D
Molecule details ›
Chains: C, D
Length: 232 amino acids
Theoretical weight: 25.24 KDa
Source organism: Psychrobacter arcticus 273-4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4FQF7 (Residues: 1-231; Coverage: 100%)
Gene names: Psyc_1903, hisG
Sequence domains: ATP phosphoribosyltransferase
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

4 bound ligands:
Ligand CL 3 x CL
Ligand ADP 2 x ADP
Ligand PRP 2 x PRP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 101.57Å b: 146.24Å c: 93.8Å
α: 90° β: 102.98° γ: 90°
R-values:
R R work R free
0.248 0.246 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links