› 6fvs

X-ray diffraction
3.2Å resolution

The active form of a pentameric ion channel (sTeLIC) gated by alkaline pH - sTeLIC in complex with Barium ions (Ba2+)

Released:
DOI: 10.2210/pdb6fvs/pdb
PDB entry 6fvs coloured by chain, front view.
PDB entry 6fvs coloured by chain, side view.
PDB entry 6fvs coloured by chain, top view.
Source organism: endosymbiont of Tevnia jerichonana (vent Tica)
Primary publication:
Crystal structures of a pentameric ion channel gated by alkaline pH show a widely open pore and identify a cavity for modulation.
Hu H, Nemecz Á, Van Renterghem C, Fourati Z, Sauguet L, Corringer PJ, Delarue M
Proc Natl Acad Sci U S A 115 E3959-E3968 (2018)
PMID: 29632192

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-515212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 320 amino acids
Theoretical weight: 37.31 KDa
Source organism: endosymbiont of Tevnia jerichonana (vent Tica)
Expression system: Escherichia coli
UniProt:
  • Canonical: G2FID1 (Residues: 1-320; Coverage: 100%)
Gene name: TevJSym_bc00020
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain

Ligands and Environments

3 bound ligands:
Ligand BNG 5 x BNG
Ligand BA 2 x BA
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 218.84Å b: 112.11Å c: 144.08Å
α: 90° β: 112.79° γ: 90°
R-values:
R R work R free
0.209 0.208 0.226
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Evolution of glutamatergic signaling and synapses.
Moroz et al. (2021)
1 more

1 mention without citation

Crystal structures of a pentameric ion channel gated by alkaline pH show a widely open pore and identify a cavity for modulation.
Hu et al. (2018)