› 6fx2

X-ray diffraction
1.7Å resolution

crystal structure of Pholiota squarrosa lectin in complex with a decasaccharide

Released:
DOI: 10.2210/pdb6fx2/pdb
PDB entry 6fx2 coloured by chain, front view.
PDB entry 6fx2 coloured by chain, side view.
PDB entry 6fx2 coloured by chain, top view.
Source organism: Pholiota squarrosa
Primary publication:
Recognition of Complex Core-Fucosylated N-Glycans by a Mini Lectin.
Cabanettes A, Perkams L, Spies C, Unverzagt C, Varrot A
Angew Chem Int Ed Engl 57 10178-10181 (2018)
PMID: 29956878

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 43 amino acids
Theoretical weight: 4.71 KDa
Source organism: Pholiota squarrosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A3B6UEU4 (Residues: 1-43; Coverage: 100%)

Ligands and Environments

Carbohydrate polymer : NEW Components: C4W, NAG, BMA, MAN, GAL, FUC
Ligand C4W 1 x C4W
Ligand NAG 3 x NAG
Ligand BMA 1 x BMA
Ligand MAN 2 x MAN
Ligand GAL 1 x GAL
Ligand FUC 1 x FUC
Carbohydrate polymer : NEW Components: C4W, NAG, BMA, MAN, GAL, FUC
Ligand C4W 1 x C4W
Ligand NAG 2 x NAG
Ligand BMA 1 x BMA
Ligand MAN 1 x MAN
Ligand GAL 1 x GAL
Ligand FUC 1 x FUC
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: I213
Unit cell:
a: 101.862Å b: 101.862Å c: 101.862Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.164 0.188
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Towards structure-focused glycoproteomics.
Chernykh et al. (2021)
7 other articles