› 6gfk

X-ray diffraction
2.3Å resolution

delta-N METTL16 MTase domain

Released:
DOI: 10.2210/pdb6gfk/pdb
PDB entry 6gfk coloured by chain, front view.
PDB entry 6gfk coloured by chain, side view.
PDB entry 6gfk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Methylation of Structured RNA by the m6A Writer METTL16 Is Essential for Mouse Embryonic Development.
Mendel M, Chen KM, Homolka D, Gos P, Pandey RR, McCarthy AA, Pillai RS
OpenAccess logo Mol Cell 71 986-1000.e11 (2018)
PMID: 30197299

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + adenine in U6 snRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in U6 snRNA
S-adenosyl-L-methionine + adenine in mRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in mRNA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-183225 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA N(6)-adenosine-methyltransferase METTL16 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 251 amino acids
Theoretical weight: 28.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86W50 (Residues: 41-291; Coverage: 45%)
Gene names: METT10D, METTL16
Sequence domains: RNA methyltransferase

Ligands and Environments

2 bound ligands:
Ligand SAH 3 x SAH
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P3121
Unit cell:
a: 133.768Å b: 133.768Å c: 78.701Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.195 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

80 review citations

Where, When, and How: Context-Dependent Functions of RNA Methylation Writers, Readers, and Erasers.
Shi et al. (2019)
79 more

2 mentions without citation

A comprehensive review of m6A/m6Am RNA methyltransferase structures.
Oerum et al. (2021)
1 more