› 6h0h

X-ray diffraction
1.39Å resolution

The ABC transporter associated binding protein from B. animalis subsp. lactis Bl-04 in complex with beta-1,6-galactobiose

Released:
DOI: 10.2210/pdb6h0h/pdb
PDB entry 6h0h coloured by chain, front view.
PDB entry 6h0h coloured by chain, side view.
PDB entry 6h0h coloured by chain, top view.
Source organism: Bifidobacterium animalis subsp. lactis Bl-04
Primary publication:
Substrate preference of an ABC importer corresponds to selective growth on β-(1,6)-galactosides in Bifidobacterium animalis subsp. lactis.
Theilmann MC, Fredslund F, Svensson B, Lo Leggio L, Abou Hachem M
J Biol Chem 294 11701-11711 (2019)
PMID: 31186348

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Probable solute binding protein of ABC transporter system for sugars Chains: A, B
Molecule details ›
Chains: A, B
Length: 426 amino acids
Theoretical weight: 46.37 KDa
Source organism: Bifidobacterium animalis subsp. lactis Bl-04
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B8DWA9 (Residues: 28-453; Coverage: 100%)
Gene name: BLA_0461
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL
Carbohydrate polymer
4 bound ligands:
Ligand GLA 2 x GLA
Ligand GAL 2 x GAL
Ligand PG4 3 x PG4
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 56.35Å b: 71.69Å c: 88.71Å
α: 90° β: 95.04° γ: 90°
R-values:
R R work R free
0.118 0.116 0.146
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Plant Glycan Metabolism by Bifidobacteria.
Kelly et al. (2021)
1 more

3 mentions without citation

Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes.
Fushinobu et al. (2021)
2 more