› 6hdg

X-ray diffraction
1.15Å resolution

D170N variant of beta-phosphoglucomutase from Lactococcus lactis complexed with beta-G1P in a closed conformer to 1.2 A.

Released:
DOI: 10.2210/pdb6hdg/pdb
PDB entry 6hdg coloured by chain, front view.
PDB entry 6hdg coloured by chain, side view.
PDB entry 6hdg coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis Il1403
Entry authors: Wood HP, Robertson AJ, Bisson C, Waltho JP

Function and Biology Details

Reaction catalysed: 
Beta-D-glucose 1-phosphate = beta-D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-phosphoglucomutase Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.24 KDa
Source organism: Lactococcus lactis subsp. lactis Il1403
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P71447 (Residues: 1-221; Coverage: 100%)
Gene names: L0001, LL0429, pgmB
Sequence domains: haloacid dehalogenase-like hydrolase

Ligands and Environments

3 bound ligands:
Ligand XGP 1 x XGP
Ligand EDO 4 x EDO
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 37.56Å b: 55.08Å c: 105.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.15 0.176
Expression system: Escherichia coli BL21(DE3)

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