› 6hk0

X-ray diffraction
3.45Å resolution

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) F16'S pore mutant (F247S) with alternate M4 conformation.

Released:
DOI: 10.2210/pdb6hk0/pdb
PDB entry 6hk0 coloured by chain, front view.
PDB entry 6hk0 coloured by chain, side view.
PDB entry 6hk0 coloured by chain, top view.
Source organism: Dickeya chrysanthemi
Primary publication:
A lipid site shapes the agonist response of a pentameric ligand-gated ion channel.
Hénault CM, Govaerts C, Spurny R, Brams M, Estrada-Mondragon A, Lynch J, Bertrand D, Pardon E, Evans GL, Woods K, Elberson BW, Cuello LG, Brannigan G, Nury H, Steyaert J, Baenziger JE, Ulens C
Nat Chem Biol 15 1156-1164 (2019)
PMID: 31591563

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cys-loop ligand-gated ion channel Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 307 amino acids
Theoretical weight: 35.36 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C7B7 (Residues: 11-163, 164-316; Coverage: 95%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain

Ligands and Environments

1 bound ligand:
Ligand LMT 10 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 105.8Å b: 264.697Å c: 111.185Å
α: 90° β: 108.8° γ: 90°
R-values:
R R work R free
0.23 0.228 0.266
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Ion channels as lipid sensors: from structures to mechanisms.
Thompson et al. (2020)
6 more

1 mention without citation

A lipid site shapes the agonist response of a pentameric ligand-gated ion channel.
Hénault et al. (2019)