6hmy

X-ray diffraction
1.6Å resolution

Cholera toxin classical B-pentamer in complex with fucosyl-GM1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
PDBe Complex ID:
PDB-CPX-134384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Cholera enterotoxin subunit B Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 103 amino acids
Theoretical weight: 11.62 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01556 (Residues: 22-124; Coverage: 100%)
Gene names: VC_1456, ctxB, toxB
Sequence domains: Heat-labile enterotoxin beta chain

Ligands and Environments

Carbohydrate polymer : NEW Components: FUC, BGC, GAL, NGA, SIA
Carbohydrate polymer : NEW Components: FUC, BGC, GAL, NGA, SIA, GLC
Carbohydrate polymer : NEW Components: FUC, BGC, GAL, NGA, SIA, GLC
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: C2
Unit cell:
a: 148.626Å b: 74.137Å c: 111.245Å
α: 90° β: 105.59° γ: 90°
R-values:
R R work R free
0.185 0.184 0.214
Expression system: Escherichia coli BL21(DE3)