› 6hno

X-ray diffraction
1.68Å resolution

17beta-hydroxysteroid dehydrogenase 14 variant S205 - mutant H93A

Released:
DOI: 10.2210/pdb6hno/pdb
PDB entry 6hno coloured by chain, front view.
PDB entry 6hno coloured by chain, side view.
PDB entry 6hno coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mutational and structural studies uncover crucial amino acids determining activity and stability of 17β-HSD14.
Badran MJ, Bertoletti N, Keils A, Heine A, Klebe G, Marchais-Oberwinkler S
J Steroid Biochem Mol Biol 189 135-144 (2019)
PMID: 30836176

Function and Biology Details

Reaction catalysed: 
A D-threo-aldose + NAD(+) = a D-threo-aldono-1,5-lactone + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-fucose dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 28.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9BPX1 (Residues: 1-270; Coverage: 100%)
Gene names: DHRS10, HSD17B14, SDR3, SDR47C1, UNQ502/PRO474
Sequence domains: Enoyl-(Acyl carrier protein) reductase

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: I422
Unit cell:
a: 91.4Å b: 91.4Å c: 132.696Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.205
Expression system: Escherichia coli BL21

Quick links

Citations

2 citation in other articles

Identification of Functional Single Nucleotide Polymorphisms in Porcine HSD17B14 Gene Associated with Estrus Behavior Difference between Large White and Mi Gilts.
Gao et al. (2020)
1 more