› 6hpf

X-ray diffraction
1.36Å resolution

Structure of Inactive E165Q mutant of fungal non-CBM carrying GH26 endo-b-mannanase from Yunnania penicillata in complex with alpha-62-61-di-galactosyl-mannotriose

Released:
DOI: 10.2210/pdb6hpf/pdb
PDB entry 6hpf coloured by chain, front view.
PDB entry 6hpf coloured by chain, side view.
PDB entry 6hpf coloured by chain, top view.
Source organism: Yunnania penicillata
Primary publication:
Crystal structure and substrate interactions of an unusual fungal non-CBM carrying GH26 endo-β-mannanase from Yunnania penicillata.
von Freiesleben P, Moroz OV, Blagova E, Wiemann M, Spodsberg N, Agger JW, Davies GJ, Wilson KS, Stålbrand H, Meyer AS, Krogh KBRM
OpenAccess logo Sci Rep 9 2266 (2019)
PMID: 30783168

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104876 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH26 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 34.49 KDa
Source organism: Yunnania penicillata
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: A0A3G4RHU4 (Residues: 19-330; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 26
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, BMA, GLA
Carbohydrate polymer
4 bound ligands:
Ligand ACY 1 x ACY
Ligand NAG 1 x NAG
Ligand SO4 6 x SO4
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P6522
Unit cell:
a: 98.988Å b: 98.988Å c: 170.497Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.123 0.122 0.143
Expression system: Aspergillus oryzae

Quick links

Citations

1 review citation

Towards an understanding of the enzymatic degradation of complex plant mannan structures.
Mafa et al. (2023)
2 other articles

5 mentions without citation

Estimation of ribosome profiling performance and reproducibility at various levels of resolution.
Diament et al. (2016)
4 more