› 6i7q

X-ray diffraction
1.8Å resolution

Structure of pVHL-elongin B-elongin C (VCB) in complex with hydroxylated-HIF-2alpha (523-542) in the C2221 form

Released:
DOI: 10.2210/pdb6i7q/pdb
PDB entry 6i7q coloured by chain, front view.
PDB entry 6i7q coloured by chain, side view.
PDB entry 6i7q coloured by chain, top view.
Source organisms:
Entry authors: Chowdhury R, Aguilera LS, Schofield CJ

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-154090 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 13.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 97 amino acids
Theoretical weight: 10.93 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2KII4 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Endothelial PAS domain-containing protein 1 Chain: H
Molecule details ›
Chain: H
Length: 20 amino acids
Theoretical weight: 2.32 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q99814 (Residues: 523-542; Coverage: 2%)
Gene names: BHLHE73, EPAS1, HIF2A, MOP2, PASD2
Sequence domains: Hypoxia-inducible factor-1
von Hippel-Lindau disease tumor suppressor Chain: V
Molecule details ›
Chain: V
Length: 160 amino acids
Theoretical weight: 18.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40337 (Residues: 54-213; Coverage: 75%)
Gene name: VHL
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
2 modified residues:
Ligand CSD 1 x CSD
Ligand HYP 1 x HYP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 81.827Å b: 86.758Å c: 136.376Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.237
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)
  • Not provided

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