› 6ibj

X-ray diffraction
2.1Å resolution

Copper binding protein from Laetisaria arvalis (LaX325)

Released:
DOI: 10.2210/pdb6ibj/pdb
PDB entry 6ibj coloured by chain, front view.
PDB entry 6ibj coloured by chain, side view.
PDB entry 6ibj coloured by chain, top view.
Source organism: Laetisaria arvalis
Primary publication:
A fungal family of lytic polysaccharide monooxygenase-like copper proteins.
Labourel A, Frandsen KEH, Zhang F, Brouilly N, Grisel S, Haon M, Ciano L, Ropartz D, Fanuel M, Martin F, Navarro D, Rosso MN, Tandrup T, Bissaro B, Johansen KS, Zerva A, Walton PH, Henrissat B, Leggio LL, Berrin JG
Nat Chem Biol 16 345-350 (2020)
PMID: 31932718

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105170 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lytic polysaccharide monooxygenase-like protein X325 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 16.66 KDa
Source organism: Laetisaria arvalis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A4P9I8G4 (Residues: 21-174; Coverage: 71%)
Gene name: X325
Sequence domains: Copper acquisition factor BIM1-like

Ligands and Environments

2 bound ligands:
Ligand NAG 4 x NAG
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P43212
Unit cell:
a: 74.92Å b: 74.92Å c: 64.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.256
Expression system: Komagataella pastoris

Quick links

Citations

4 review citations

On the impact of carbohydrate-binding modules (CBMs) in lytic polysaccharide monooxygenases (LPMOs).
Forsberg et al. (2023)
3 more

4 mentions without citation

Lytic polysaccharide monooxygenases and other histidine-brace copper proteins: structure, oxygen activation and biotechnological applications.
Ipsen et al. (2021)
3 more