› 6idj

X-ray diffraction
1.9Å resolution

Crystal structure of human DHODH in complex with ferulenol

Released:
DOI: 10.2210/pdb6idj/pdb
PDB entry 6idj coloured by chain, front view.
PDB entry 6idj coloured by chain, side view.
PDB entry 6idj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and Biochemical Features of Eimeria tenella Dihydroorotate Dehydrogenase, a Potential Drug Target.
Sato D, Hartuti ED, Inaoka DK, Sakura T, Amalia E, Nagahama M, Yoshioka Y, Tsuji N, Nozaki T, Kita K, Harada S, Matsubayashi M, Shiba T
OpenAccess logo Genes (Basel) 11 (2020)
PMID: 33297567

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + a quinone = orotate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-554201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase (quinone), mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 390 amino acids
Theoretical weight: 42.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02127 (Residues: 29-395; Coverage: 93%)
Gene name: DHODH
Sequence domains: Dihydroorotate dehydrogenase

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
4 bound ligands:
Ligand SO4 3 x SO4
Ligand ACT 3 x ACT
Ligand ORO 1 x ORO
Ligand 9AU 1 x 9AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P3221
Unit cell:
a: 90.155Å b: 90.155Å c: 123.334Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.163 0.178
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Biochemical Studies of Mitochondrial Malate: Quinone Oxidoreductase from Toxoplasma gondii.
Acharjee et al. (2021)
3 more

3 mentions without citation

Regulation and functional role of the electron transport chain supercomplexes.
Cogliati et al. (2021)
2 more