› 6ifa

X-ray diffraction
1.9Å resolution

Structure of beta-trefoil lectin from Entamoeba histolytica

Released:
DOI: 10.2210/pdb6ifa/pdb
PDB entry 6ifa coloured by chain, front view.
PDB entry 6ifa coloured by chain, side view.
PDB entry 6ifa coloured by chain, top view.
Source organism: Entamoeba histolytica HM-1:IMSS
Primary publication:
Crystal structures of a β-trefoil lectin from Entamoeba histolytica in monomeric and a novel disulfide bond-mediated dimeric forms.
Khan F, Kurre D, Suguna K
Glycobiology 30 474-488 (2020)
PMID: 31967310

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-517091 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin B lectin domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 129 amino acids
Theoretical weight: 14.68 KDa
Source organism: Entamoeba histolytica HM-1:IMSS
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: N9TFI9 (Residues: 1-129; Coverage: 100%)
Gene name: EHI7A_061220
Sequence domains: Ricin-type beta-trefoil lectin domain

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand IPA 9 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: I422
Unit cell:
a: 101.732Å b: 101.732Å c: 183.996Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.236
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

1 review citation

Pathogenicity and virulence of Entamoeba histolytica, the agent of amoebiasis.
Guillén N. (2023)
1 other article