› 6ifb

X-ray diffraction
1.37Å resolution

Structure of rhamnose bound beta-trefoil lectin from Entamoeba histolytica

Released:
DOI: 10.2210/pdb6ifb/pdb
PDB entry 6ifb coloured by chain, front view.
PDB entry 6ifb coloured by chain, side view.
PDB entry 6ifb coloured by chain, top view.
Source organism: Entamoeba histolytica HM-1:IMSS
Primary publication:
Crystal structures of a β-trefoil lectin from Entamoeba histolytica in monomeric and a novel disulfide bond-mediated dimeric forms.
Khan F, Kurre D, Suguna K
Glycobiology 30 474-488 (2020)
PMID: 31967310

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin B lectin domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.31 KDa
Source organism: Entamoeba histolytica HM-1:IMSS
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: N9TFI9 (Residues: 1-129; Coverage: 100%)
Gene name: EHI7A_061220
Sequence domains: Ricin-type beta-trefoil lectin domain

Ligands and Environments

3 bound ligands:
Ligand RM4 2 x RM4
Ligand GOL 2 x GOL
Ligand IPA 3 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 57.194Å b: 62.377Å c: 67.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.158 0.177
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

Pathogenicity and virulence of Entamoeba histolytica, the agent of amoebiasis.
Guillén N. (2023)
1 other article

1 mention without citation

Streamlining N-terminally anchored yeast surface display via structural insights into S. cerevisiae Pir proteins.
Martinić Cezar et al. (2023)