› 6ii9

X-ray diffraction
3.5Å resolution

Crystal structure of H7 hemagglutinin from A/Anhui/1/2013 in complex with a human neutralizing antibody L3A-44

Released:
DOI: 10.2210/pdb6ii9/pdb
PDB entry 6ii9 coloured by chain, front view.
PDB entry 6ii9 coloured by chain, side view.
PDB entry 6ii9 coloured by chain, top view.
Source organisms:
Primary publication:
Structure-function analysis of neutralizing antibodies to H7N9 influenza from naturally infected humans.
Huang KA, Rijal P, Jiang H, Wang B, Schimanski L, Dong T, Liu YM, Chang P, Iqbal M, Wang MC, Chen Z, Song R, Huang CC, Yang JH, Qi J, Lin TY, Li A, Powell TJ, Jan JT, Ma C, Gao GF, Shi Y, Townsend AR
Nat Microbiol 4 306-315 (2019)
PMID: 30478290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-492245 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 317 amino acids
Theoretical weight: 34.55 KDa
Source organism: Influenza A virus (A/Anhui/DEWH72-02/2013(H7N9))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A024CX39 (Residues: 19-335; Coverage: 57%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 170 amino acids
Theoretical weight: 19.65 KDa
Source organism: Influenza A virus (A/Anhui/1-YK_RG123/2013(H7N9))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A0K1LUI9 (Residues: 340-509; Coverage: 30%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
Heavy chain of L3A-44 Fab Chains: H, M, P
Molecule details ›
Chains: H, M, P
Length: 221 amino acids
Theoretical weight: 23.41 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Light chain of L3A-44 Fab Chains: L, N, Q
Molecule details ›
Chains: L, N, Q
Length: 212 amino acids
Theoretical weight: 22.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 178.457Å b: 184.906Å c: 188.99Å
α: 90° β: 106.47° γ: 90°
R-values:
R R work R free
0.202 0.201 0.234
Expression system: Spodoptera frugiperda

Quick links

Citations

5 review citations

How repertoire data are changing antibody science.
Marks et al. (2020)
4 more

3 mentions without citation

Affinity maturation is required for pathogenic monovalent IgG4 autoantibody development in myasthenia gravis.
Fichtner et al. (2020)
2 more