› 6iqg

X-ray diffraction
3Å resolution

X-ray crystal structure of Fc and peptide complex

Released:
DOI: 10.2210/pdb6iqg/pdb
PDB entry 6iqg coloured by chain, front view.
PDB entry 6iqg coloured by chain, side view.
PDB entry 6iqg coloured by chain, top view.
Source organisms:
Primary publication:
Site-Specific Chemical Conjugation of Antibodies by Using Affinity Peptide for the Development of Therapeutic Antibody Format.
Kishimoto S, Nakashimada Y, Yokota R, Hatanaka T, Adachi M, Ito Y
Bioconjug Chem 30 698-702 (2019)
PMID: 30606013

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-534184 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin gamma-1 heavy chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 23.79 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P0DOX5 (Residues: 238-447; Coverage: 47%)
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
18-mer peptide G(HCS)DCAYHRGELVWCT(HCS)H(NH2) Chains: C, D
Molecule details ›
Chains: C, D
Length: 18 amino acids
Theoretical weight: 1.98 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 66.062Å b: 60.507Å c: 69.526Å
α: 90° β: 101.25° γ: 90°
R-values:
R R work R free
0.202 0.198 0.273
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

5 review citations

Precision Modification of Native Antibodies.
Wu et al. (2021)
4 more

2 mentions without citation

Site-selective lysine conjugation methods and applications towards antibody-drug conjugates.
Haque et al. (2021)
1 more