› 6iuv

X-ray diffraction
2.33Å resolution

Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody 3C11

Released:
DOI: 10.2210/pdb6iuv/pdb
PDB entry 6iuv coloured by chain, front view.
PDB entry 6iuv coloured by chain, side view.
PDB entry 6iuv coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional definition of a vulnerable site on the hemagglutinin of highly pathogenic avian influenza A virus H5N1.
Wang P, Zuo Y, Sun J, Zuo T, Zhang S, Guo S, Shi X, Liang M, Zhou P, Zhang L, Wang X
J Biol Chem 294 4290-4303 (2019)
PMID: 30737282

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212458 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 25.44 KDa
Source organism: Influenza A virus (A/Hong Kong/482/97(H5N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q77XR4 (Residues: 61-284; Coverage: 39%)
Gene names: H5, HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1
3C11 Light Chain Chains: D, L
Molecule details ›
Chains: D, L
Length: 218 amino acids
Theoretical weight: 23.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
3C11 Heavy Chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 229 amino acids
Theoretical weight: 24.66 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 126.78Å b: 62.236Å c: 127.827Å
α: 90° β: 117.21° γ: 90°
R-values:
R R work R free
0.209 0.207 0.246
Expression systems:
  • Spodoptera frugiperda
  • Homo sapiens

Quick links

Citations

2 review citations

Targeting Hemagglutinin: Approaches for Broad Protection against the Influenza A Virus.
Zhang et al. (2019)
1 more

1 mention without citation

Structural and functional definition of a vulnerable site on the hemagglutinin of highly pathogenic avian influenza A virus H5N1.
Wang et al. (2019)