› 6jao

X-ray diffraction
1.77Å resolution

Crystal structure of ABC transporter alpha-glycoside-binding mutant protein R356A in complex with palatinose

Released:
DOI: 10.2210/pdb6jao/pdb
PDB entry 6jao coloured by chain, front view.
PDB entry 6jao coloured by chain, side view.
PDB entry 6jao coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structural and thermodynamic correlation illuminates the selective transport mechanism of disaccharide α-glycosides through ABC transporter.
Chandravanshi M, Gogoi P, Kanaujia SP
FEBS J 287 1576-1597 (2020)
PMID: 31608555

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
ABC transporter, periplasmic substrate-binding protein Chain: A
Molecule details ›
Chain: A
Length: 408 amino acids
Theoretical weight: 46.08 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SLD7 (Residues: 28-429; Coverage: 100%)
Gene name: TTHA0356
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: Z9N, GLC
Carbohydrate polymer
3 bound ligands:
Ligand CIT 1 x CIT
Ligand EDO 1 x EDO
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P41212
Unit cell:
a: 85.42Å b: 85.42Å c: 146.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.199
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Binding modes of methyl α-d-glucopyranoside to an artificial receptor in crystalline complexes.
Köhler et al. (2021)
4 more

1 mention without citation

Structural and thermodynamic correlation illuminates the selective transport mechanism of disaccharide α-glycosides through ABC transporter.
Chandravanshi et al. (2020)