› 6jr8

X-ray diffraction
1.8Å resolution

Flavobacterium johnsoniae GH31 dextranase, FjDex31A, mutant D412A complexed with isomaltotriose

Released:
DOI: 10.2210/pdb6jr8/pdb
PDB entry 6jr8 coloured by chain, front view.
PDB entry 6jr8 coloured by chain, side view.
PDB entry 6jr8 coloured by chain, top view.
Source organism: Flavobacterium johnsoniae UW101
Primary publication:
Structural insights into polysaccharide recognition by Flavobacterium johnsoniae dextranase, a member of glycoside hydrolase family 31.
Tsutsumi K, Gozu Y, Nishikawa A, Tonozuka T
FEBS J 287 1195-1207 (2020)
PMID: 31552702

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Candidate alpha-glycosidase Glycoside hydrolase family 31 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 838 amino acids
Theoretical weight: 96.14 KDa
Source organism: Flavobacterium johnsoniae UW101
Expression system: Escherichia coli
UniProt:
  • Canonical: A5FBI1 (Residues: 21-836; Coverage: 100%)
Gene name: Fjoh_4430
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
2 bound ligands:
Ligand ACT 4 x ACT
Ligand MPD 13 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P1
Unit cell:
a: 102.37Å b: 112.625Å c: 114.172Å
α: 111.06° β: 92.98° γ: 114.54°
R-values:
R R work R free
0.151 0.15 0.174
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Marine Bacterial Dextranases: Fundamentals and Applications.
Barzkar et al. (2022)
4 other articles

1 mention without citation

Structural insights into polysaccharide recognition by Flavobacterium johnsoniae dextranase, a member of glycoside hydrolase family 31.
Tsutsumi et al. (2020)