6krv

X-ray diffraction
3.3Å resolution

Crystal structure of mouse IgG2b Fc complexed with B domain of Protein A

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-134932 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin heavy constant gamma 2B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 218 amino acids
Theoretical weight: 24.53 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01867 (Residues: 117-334; Coverage: 54%)
Gene names: Igh-3, Ighg2b
Sequence domains: Immunoglobulin C1-set domain
Immunoglobulin G-binding protein A Chain: D
Molecule details ›
Chain: D
Length: 58 amino acids
Theoretical weight: 6.61 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38507 (Residues: 212-269; Coverage: 12%)
Gene name: spa
Sequence domains: B domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN, FUC
Carbohydrate polymer : NEW Components: NAG, MAN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P42212
Unit cell:
a: 145.28Å b: 145.28Å c: 155.528Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.25
Expression system: Escherichia coli BL21(DE3)