› 6kwg

X-ray diffraction
1.69Å resolution

Crystal Structure Analysis of Endo-beta-1,4-xylanase II Complexed with Xylotriose

Released:
DOI: 10.2210/pdb6kwg/pdb
PDB entry 6kwg coloured by chain, front view.
PDB entry 6kwg coloured by chain, side view.
PDB entry 6kwg coloured by chain, top view.
Source organism: Trichoderma reesei RUT C-30
Primary publication:
Studying the Role of a Single Mutation of a Family 11 Glycoside Hydrolase Using High-Resolution X-ray Crystallography.
Li Z, Zhang X, Li C, Kovalevsky A, Wan Q
Protein J 39 671-680 (2020)
PMID: 33128114

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endo-1,4-beta-xylanase 2 Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 20.74 KDa
Source organism: Trichoderma reesei RUT C-30
Expression system: Escherichia coli
UniProt:
  • Canonical: P36217 (Residues: 35-223; Coverage: 93%)
Gene names: M419DRAFT_124931, xyn2
Sequence domains: Glycosyl hydrolases family 11

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
1 bound ligand:
Ligand IOD 7 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 46.915Å b: 58.533Å c: 69.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.244
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Cloning and Heterologous Expression of a Novel Xylanase Gene TAX1 from Trichoderma atroviride and Its Application in the Deconstruction of Corn Stover.
Cai et al. (2021)