› 6l6r

X-ray diffraction
3.8Å resolution

Crystal structure of LRP6 E1E2-SOST complex

Released:
DOI: 10.2210/pdb6l6r/pdb
PDB entry 6l6r coloured by chain, front view.
PDB entry 6l6r coloured by chain, side view.
PDB entry 6l6r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Sclerostin inhibits Wnt signaling through tandem interaction with two LRP6 ectodomains.
Kim J, Han W, Park T, Kim EJ, Bang I, Lee HS, Jeong Y, Roh K, Kim J, Kim JS, Kang C, Seok C, Han JK, Choi HJ
OpenAccess logo Nat Commun 11 5357 (2020)
PMID: 33097721

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131066 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Low-density lipoprotein receptor-related protein 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 622 amino acids
Theoretical weight: 70.53 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O75581 (Residues: 21-630; Coverage: 38%)
Gene name: LRP6
Sequence domains:
Sclerostin Chains: C, D
Molecule details ›
Chains: C, D
Length: 158 amino acids
Theoretical weight: 17.91 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9BQB4 (Residues: 24-177; Coverage: 81%)
Gene names: SOST, UNQ2976/PRO7455/PRO7476
Sequence domains: Sclerostin (SOST)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
3 bound ligands:
Ligand NAG 2 x NAG
Ligand MG 4 x MG
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P64
Unit cell:
a: 252.792Å b: 252.792Å c: 86.286Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.207 0.256
Expression system: Trichoplusia ni

Quick links

Citations

11 review citations

LRPs in WNT Signalling.
Davidson G. (2021)
10 more

5 mentions without citation

Sclerostin: From Molecule to Clinical Biomarker.
Omran et al. (2022)
4 more