› 6lf3

X-ray diffraction
3.2Å resolution

3D domain-swapped dimer of the maltose-binding protein fused to a fragment of the protein-tyrosine kinase 2-beta

Released:
DOI: 10.2210/pdb6lf3/pdb
PDB entry 6lf3 coloured by chain, front view.
PDB entry 6lf3 coloured by chain, side view.
PDB entry 6lf3 coloured by chain, top view.
Source organisms:
Primary publication:
Passenger sequences can promote interlaced dimers in a common variant of the maltose-binding protein.
Momin AA, Hameed UFS, Arold ST
OpenAccess logo Sci Rep 9 20396 (2019)
PMID: 31892719

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Protein-tyrosine kinase 2-beta Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 419 amino acids
Theoretical weight: 46.4 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: Q14289 (Residues: 790-839; Coverage: 5%)
Gene names: FAK2, JW3994, PTK2B, PYK2, RAFTK, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P1
Unit cell:
a: 78.78Å b: 92.74Å c: 93.61Å
α: 112.46° β: 101.66° γ: 94.93°
R-values:
R R work R free
0.246 0.244 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Autoimmune diseases: targets, biology, and drug discovery.
Li et al. (2023)
4 other articles