6lpb

Electron Microscopy
3.9Å resolution

Cryo-EM structure of the human PAC1 receptor coupled to an engineered heterotrimeric G protein

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-538946 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Pituitary adenylate cyclase-activating polypeptide 38 Chain: P
Molecule details ›
Chain: P
Length: 38 amino acids
Theoretical weight: 4.55 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P18509 (Residues: 132-169; Coverage: 25%)
Gene name: ADCYAP1
Sequence domains: Peptide hormone
Pituitary adenylate cyclase-activating polypeptide type I receptor Chain: R
Molecule details ›
Chain: R
Length: 403 amino acids
Theoretical weight: 46.68 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P41586 (Residues: 21-417; Coverage: 89%)
Gene name: ADCYAP1R1
Sequence domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
nanobody Nb35 Chain: N
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-0940
Expression systems:
  • Not provided
  • Homo sapiens
  • Spodoptera frugiperda
  • Escherichia coli