› 6lzv

X-ray diffraction
2.2Å resolution

F437A mutant of chitin-specific solute binding protein from Vibrio harveyi co-crystalized with chitobiose.

Released:
DOI: 10.2210/pdb6lzv/pdb
PDB entry 6lzv coloured by chain, front view.
PDB entry 6lzv coloured by chain, side view.
PDB entry 6lzv coloured by chain, top view.
Source organism: Vibrio harveyi 1DA3
Primary publication:
A structural model for (GlcNAc)2 translocation via a periplasmic chitooligosaccharide-binding protein from marine Vibrio bacteria.
Kitaoku Y, Fukamizo T, Kumsaoad S, Ubonbal P, Robinson RC, Suginta W
OpenAccess logo J Biol Chem 297 101071 (2021)
PMID: 34400168

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237336 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Peptide ABC transporter, periplasmic peptide-binding protein Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 61.21 KDa
Source organism: Vibrio harveyi 1DA3
Expression system: Escherichia coli

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand EDO 1 x EDO
Ligand CL 3 x CL
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE TPS 05A
Spacegroup: P21
Unit cell:
a: 59.522Å b: 56.957Å c: 85.152Å
α: 90° β: 101.36° γ: 90°
R-values:
R R work R free
0.174 0.171 0.225
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Periplasmic chitooligosaccharide-binding protein requires a three-domain organization for substrate translocation.
Ohnuma et al. (2023)

1 mention without citation

A structural model for (GlcNAc)2 translocation via a periplasmic chitooligosaccharide-binding protein from marine Vibrio bacteria.
Kitaoku et al. (2021)