› 6msy

X-ray diffraction
2Å resolution

Anti-HIV-1 Fab Fab 2G12 + Man4 re-refinement

Released:
DOI: 10.2210/pdb6msy/pdb
PDB entry 6msy coloured by chain, front view.
PDB entry 6msy coloured by chain, side view.
PDB entry 6msy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dissection of the carbohydrate specificity of the broadly neutralizing anti-HIV-1 antibody 2G12.
Calarese DA, Lee HK, Huang CY, Best MD, Astronomo RD, Stanfield RL, Katinger H, Burton DR, Wong CH, Wilson IA
Proc Natl Acad Sci U S A 102 13372-7 (2005)
PMID: 16174734

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143690 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin kappa light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.25 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P0DOX7 (Residues: 1-213; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
Immunoglobulin gamma-1 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 23.93 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P0DOX5 (Residues: 1-98, 99-221; Coverage: 49%)
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
2 bound ligands:
Ligand UNX 4 x UNX
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C222
Unit cell:
a: 144.646Å b: 148.319Å c: 54.604Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.242
Expression system: Cricetulus griseus

Quick links

Citations

48 review citations

Virus glycosylation: role in virulence and immune interactions.
Vigerust et al. (2007)
47 more

2 mentions without citation

Fast Quantitative Validation of 3D Models of Low-Affinity Protein-Ligand Complexes by STD NMR Spectroscopy.
Nepravishta et al. (2024)
1 more