6n50

X-ray diffraction
3.75Å resolution

Metabotropic Glutamate Receptor 5 Extracellular Domain in Complex with Nb43 and L-quisqualic acid

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
PDBe Complex ID:
PDB-CPX-231807 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Metabotropic glutamate receptor 5 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 596 amino acids
Theoretical weight: 67.4 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P41594 (Residues: 21-571; Coverage: 46%)
Gene names: GPRC1E, GRM5, MGLUR5
Sequence domains:
Nanobody 43 Chain: E
Molecule details ›
Chain: E
Length: 123 amino acids
Theoretical weight: 13.35 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I212121
Unit cell:
a: 150.433Å b: 157.159Å c: 208.112Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.266 0.3
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21(DE3)