› 6n86

X-ray diffraction
3.9Å resolution

Resistance to inhibitors of cholinesterase 8A (Ric8A) protein

Released:
DOI: 10.2210/pdb6n86/pdb
PDB entry 6n86 coloured by chain, front view.
PDB entry 6n86 coloured by chain, side view.
PDB entry 6n86 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structural underpinnings of Ric8A function as a G-protein α-subunit chaperone and guanine-nucleotide exchange factor.
Srivastava D, Gakhar L, Artemyev NO
OpenAccess logo Nat Commun 10 3084 (2019)
PMID: 31300652

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177006 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperone Ric-8A Chain: B
Molecule details ›
Chain: B
Length: 512 amino acids
Theoretical weight: 57.64 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5E9J8 (Residues: 1-492; Coverage: 93%)
Gene name: RIC8A
Sequence domains: Guanine nucleotide exchange factor synembryn

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: F222
Unit cell:
a: 95.15Å b: 134.34Å c: 221.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.256 0.296
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Strategies towards Targeting Gαi/s Proteins: Scanning of Protein-Protein Interaction Sites To Overcome Inaccessibility.
Nubbemeyer et al. (2021)
9 other articles

1 mention without citation

Ric-8A, a GEF, and a Chaperone for G Protein α-Subunits: Evidence for the Two-Faced Interface.
Srivastava et al. (2020)