› 6nsm

X-ray diffraction
2.8Å resolution

TetR family transcriptional regulator CifR C99T-C107S-C181R Cysteines mutant complexed with 26bp double-strand operator DNA

Released:
DOI: 10.2210/pdb6nsm/pdb
PDB entry 6nsm coloured by chain, front view.
PDB entry 6nsm coloured by chain, side view.
PDB entry 6nsm coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for the transcriptional regulation of an epoxide-based virulence circuit in Pseudomonas aeruginosa.
He S, Taher NM, Simard AR, Hvorecny KL, Ragusa MJ, Bahl CD, Hickman AB, Dyda F, Madden DR
Nucleic Acids Res (2024)
PMID: 39413156

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-120352 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 21.97 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HZR6 (Residues: 1-196; Coverage: 100%)
Gene names: PA2931, cifR
Sequence domains:
DNA (26-MER) Chain: C
Molecule details ›
Chain: C
Length: 26 nucleotides
Theoretical weight: 7.94 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided
DNA (26-MER) Chain: D
Molecule details ›
Chain: D
Length: 26 nucleotides
Theoretical weight: 8.03 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-2
Spacegroup: P21212
Unit cell:
a: 64.329Å b: 167.897Å c: 82.538Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.248
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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