› 6nyq

X-ray diffraction
1.85Å resolution

Crystal structure of glycosylated lysosomal membrane protein (GLMP) luminal domain bound to a Fab fragment

Released:
DOI: 10.2210/pdb6nyq/pdb
PDB entry 6nyq coloured by chain, front view.
PDB entry 6nyq coloured by chain, side view.
PDB entry 6nyq coloured by chain, top view.
Source organism: Mus musculus
Entry authors: Huang CS, Boenig G, Hymowitz SG

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212763 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
1H3 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.57 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
1H3 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 227 amino acids
Theoretical weight: 24.07 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Glycosylated lysosomal membrane protein Chain: C
Molecule details ›
Chain: C
Length: 404 amino acids
Theoretical weight: 43.83 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9JHJ3 (Residues: 1-404; Coverage: 100%)
Gene name: Glmp
Sequence domains: Lysosomal transcription factor, NCU-G1

Ligands and Environments

2 bound ligands:
Ligand NA 4 x NA
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 81.924Å b: 55.238Å c: 99.639Å
α: 90° β: 99.35° γ: 90°
R-values:
R R work R free
0.166 0.164 0.204
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda

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